Review



empty vector ev expression plasmid  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc empty vector ev expression plasmid
    Empty Vector Ev Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pMax-E2F1+(Plasmid+%2316007)/10__1158_slash_2767___9764__crc___23___0370-46-3-12
    Average 93 stars, based on 18 article reviews
    empty vector ev expression plasmid - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Luciferase:

    Article Title: Activation of microRNA-378a-3p biogenesis promotes hepatic secretion of VLDL and hyperlipidemia by modulating ApoB100-Sortilin1 axis
    Article Snippet: .. Then, 200 ng of the luciferase reporter vector and E2f1 expression vector (pCDNA3.1-E2F1, Addgene, 200 ng) as well as 30 ng of β-gal plasmid pSV-β-Galactosidase were transfected into Hepa1-6 cells using Lipofectamine 2000 (Invitrogen). ..

    Expressing:

    Article Title: Activation of microRNA-378a-3p biogenesis promotes hepatic secretion of VLDL and hyperlipidemia by modulating ApoB100-Sortilin1 axis
    Article Snippet: .. Then, 200 ng of the luciferase reporter vector and E2f1 expression vector (pCDNA3.1-E2F1, Addgene, 200 ng) as well as 30 ng of β-gal plasmid pSV-β-Galactosidase were transfected into Hepa1-6 cells using Lipofectamine 2000 (Invitrogen). ..

    Article Title: miR-302b enhances breast cancer cell sensitivity to cisplatin by regulating E2F1 and the cellular DNA damage response.
    Article Snippet: .. The E2F1 expression vector was purchased from Addgene (Plasmid #21667) [46]. .. Luciferase assays for target and promoter identification Luciferase reporter vectors (see above) and 10 ng of the pRL-SV40 (Renilla) control vector (Promega), and 100 nM miR-302b or scrambled sequence miRNA control (Ambion Inc, Austin, TX) were co-transfected into MDA-MB-231 cells in 12-well plates.

    Article Title: Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1
    Article Snippet: .. CaSki and HeLa cells were also transfected with 2 μg of an E2F1 expression vector (pRcCMV-HA-E2F1; Addgene, Watertown, MA) with X-tremeGENE HP DNA transfection reagent (Roche), and total RNA from the cells with E2F1 overexpression was analyzed for RNASEH2A expression by real-time RT-qPCR. ..

    Article Title: miR-302b enhances breast cancer cell sensitivity to cisplatin by regulating E2F1 and the cellular DNA damage response
    Article Snippet: .. The E2F1 expression vector was purchased from Addgene (Plasmid #21667) [ ]. .. Luciferase reporter vectors (see above) and 10 ng of the pRL-SV40 (Renilla) control vector (Promega), and 100 nM miR-302b or scrambled sequence miRNA control (Ambion Inc, Austin, TX) were co-transfected into MDA-MB-231 cells in 12-well plates.

    Article Title: Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1
    Article Snippet: .. CaSki and HeLa cells were also transfected with 2 g of an E2F1 expression vector (pRcCMV-HA-E2F1; Addgene, Watertown, MA) with X-tremeGENE HP DNA transfection reagent (Roche), and total RNA from the cells with E2F1 overexpression was analyzed for RNASEH2A expression by real-time RT-qPCR. ..

    Plasmid Preparation:

    Article Title: Activation of microRNA-378a-3p biogenesis promotes hepatic secretion of VLDL and hyperlipidemia by modulating ApoB100-Sortilin1 axis
    Article Snippet: .. Then, 200 ng of the luciferase reporter vector and E2f1 expression vector (pCDNA3.1-E2F1, Addgene, 200 ng) as well as 30 ng of β-gal plasmid pSV-β-Galactosidase were transfected into Hepa1-6 cells using Lipofectamine 2000 (Invitrogen). ..

    Article Title: miR-302b enhances breast cancer cell sensitivity to cisplatin by regulating E2F1 and the cellular DNA damage response.
    Article Snippet: .. The E2F1 expression vector was purchased from Addgene (Plasmid #21667) [46]. .. Luciferase assays for target and promoter identification Luciferase reporter vectors (see above) and 10 ng of the pRL-SV40 (Renilla) control vector (Promega), and 100 nM miR-302b or scrambled sequence miRNA control (Ambion Inc, Austin, TX) were co-transfected into MDA-MB-231 cells in 12-well plates.

    Article Title: miR-302b enhances breast cancer cell sensitivity to cisplatin by regulating E2F1 and the cellular DNA damage response
    Article Snippet: .. The E2F1 expression vector was purchased from Addgene (Plasmid #21667) [ ]. .. Luciferase reporter vectors (see above) and 10 ng of the pRL-SV40 (Renilla) control vector (Promega), and 100 nM miR-302b or scrambled sequence miRNA control (Ambion Inc, Austin, TX) were co-transfected into MDA-MB-231 cells in 12-well plates.

    Transfection:

    Article Title: Activation of microRNA-378a-3p biogenesis promotes hepatic secretion of VLDL and hyperlipidemia by modulating ApoB100-Sortilin1 axis
    Article Snippet: .. Then, 200 ng of the luciferase reporter vector and E2f1 expression vector (pCDNA3.1-E2F1, Addgene, 200 ng) as well as 30 ng of β-gal plasmid pSV-β-Galactosidase were transfected into Hepa1-6 cells using Lipofectamine 2000 (Invitrogen). ..

    Article Title: Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1
    Article Snippet: .. CaSki and HeLa cells were also transfected with 2 μg of an E2F1 expression vector (pRcCMV-HA-E2F1; Addgene, Watertown, MA) with X-tremeGENE HP DNA transfection reagent (Roche), and total RNA from the cells with E2F1 overexpression was analyzed for RNASEH2A expression by real-time RT-qPCR. ..

    Article Title: Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1
    Article Snippet: .. CaSki and HeLa cells were also transfected with 2 g of an E2F1 expression vector (pRcCMV-HA-E2F1; Addgene, Watertown, MA) with X-tremeGENE HP DNA transfection reagent (Roche), and total RNA from the cells with E2F1 overexpression was analyzed for RNASEH2A expression by real-time RT-qPCR. ..

    Over Expression:

    Article Title: Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1
    Article Snippet: .. CaSki and HeLa cells were also transfected with 2 μg of an E2F1 expression vector (pRcCMV-HA-E2F1; Addgene, Watertown, MA) with X-tremeGENE HP DNA transfection reagent (Roche), and total RNA from the cells with E2F1 overexpression was analyzed for RNASEH2A expression by real-time RT-qPCR. ..

    Article Title: Genome-Wide Profiling of Cervical RNA-Binding Proteins Identifies Human Papillomavirus Regulation of RNASEH2A Expression by Viral E7 and E2F1
    Article Snippet: .. CaSki and HeLa cells were also transfected with 2 g of an E2F1 expression vector (pRcCMV-HA-E2F1; Addgene, Watertown, MA) with X-tremeGENE HP DNA transfection reagent (Roche), and total RNA from the cells with E2F1 overexpression was analyzed for RNASEH2A expression by real-time RT-qPCR. ..



    Similar Products

    empty vector ev expression plasmid  (Addgene inc)
    93
    Addgene inc empty vector ev expression plasmid
    Empty Vector Ev Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pMax-E2F1+(Plasmid+%2316007)/10__1158_slash_2767___9764__crc___23___0370-46-3-12
    Average 93 stars, based on 1 article reviews
    empty vector ev expression plasmid - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    empty vector expression plasmid  (Addgene inc)
    93
    Addgene inc empty vector expression plasmid
    Empty Vector Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pMax-E2F1+(Plasmid+%2316007)/pmc11288008-44-3-11
    Average 93 stars, based on 1 article reviews
    empty vector expression plasmid - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    pmax e2f1 expression vector  (Addgene inc)
    93
    Addgene inc pmax e2f1 expression vector
    <t>E2F1</t> upregulates LSINCT5 expression in GC cells. Notes: qRT-PCR analysis of the E2F1 expression levels in GC tissues and paired ANTs ( A ) and in metastatic and nonmetastatic GC tissues ( B ). ( C ) qRT-PCR analysis of E2F1 expression in a series of human GC cell lines and a normal gastric epithelial cell line (GES-1). ( D ) ChIP assays using E2F1 antibody demonstrated endogenous E2F1 binding to the LSINCT5 gene promoter, and ectopic expression or siRNA knockdown increased or reduced E2F1 enrichment at the LSINCT5 promoter, respectively. ( E ) A dual-luciferase reporter assay was performed through cotransfection of the LSINCT5 promoter fragment (pGL3-LSINCT5) and an E2F1-overexpression construct. ( F ) We examined the LSINCT5 core promoter region for transcription factor binding sites, and identified four tandem putative E2F1-binding sites. Reporter assays were conducted in cells transfected with various LSINCT5 promoter constructs in which different E2F1-binding elements were deleted (WT, del). Luciferase activity is presented relative to that of the pGL3 vector (a promoter-less vector). ( G ) qRT-PCR analysis of the LSINCT5 expression levels following the transfection of MGC803 and BGC823 cells with a <t>pMax-E2F1</t> expression vector and siRNA-E2F1, respectively. ( H ) Analysis of the relationship between LSINCT5 and E2F1 mRNA levels (ΔCt value) in GC tissues. Bars: SD; * P <0.05 and ** P <0.01. Abbreviations: ANTs, adjacent normal tissues; Ts, tumor tissues; ChIP, chromatin immunoprecipitation; del, deletion; GC, gastric cancer; qRT-PCR, quantitative real-time polymerase chain reaction; WT, wild type; NC, negative control.
    Pmax E2f1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pMax-E2F1+(Plasmid+%2316007)/pmc06089107-40-1-7
    Average 93 stars, based on 1 article reviews
    pmax e2f1 expression vector - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    e2f1 expression vector pcmvha e2f1  (Addgene inc)
    94
    Addgene inc e2f1 expression vector pcmvha e2f1
    <t>E2F1</t> upregulates LSINCT5 expression in GC cells. Notes: qRT-PCR analysis of the E2F1 expression levels in GC tissues and paired ANTs ( A ) and in metastatic and nonmetastatic GC tissues ( B ). ( C ) qRT-PCR analysis of E2F1 expression in a series of human GC cell lines and a normal gastric epithelial cell line (GES-1). ( D ) ChIP assays using E2F1 antibody demonstrated endogenous E2F1 binding to the LSINCT5 gene promoter, and ectopic expression or siRNA knockdown increased or reduced E2F1 enrichment at the LSINCT5 promoter, respectively. ( E ) A dual-luciferase reporter assay was performed through cotransfection of the LSINCT5 promoter fragment (pGL3-LSINCT5) and an E2F1-overexpression construct. ( F ) We examined the LSINCT5 core promoter region for transcription factor binding sites, and identified four tandem putative E2F1-binding sites. Reporter assays were conducted in cells transfected with various LSINCT5 promoter constructs in which different E2F1-binding elements were deleted (WT, del). Luciferase activity is presented relative to that of the pGL3 vector (a promoter-less vector). ( G ) qRT-PCR analysis of the LSINCT5 expression levels following the transfection of MGC803 and BGC823 cells with a <t>pMax-E2F1</t> expression vector and siRNA-E2F1, respectively. ( H ) Analysis of the relationship between LSINCT5 and E2F1 mRNA levels (ΔCt value) in GC tissues. Bars: SD; * P <0.05 and ** P <0.01. Abbreviations: ANTs, adjacent normal tissues; Ts, tumor tissues; ChIP, chromatin immunoprecipitation; del, deletion; GC, gastric cancer; qRT-PCR, quantitative real-time polymerase chain reaction; WT, wild type; NC, negative control.
    E2f1 Expression Vector Pcmvha E2f1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pCMVHA+E2F1+(Plasmid+%2324225)/pm32354165-77-11-22
    Average 94 stars, based on 1 article reviews
    e2f1 expression vector pcmvha e2f1 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    e2f1 expression vector pcmv-e2f1  (Thermo Fisher)
    90
    Thermo Fisher e2f1 expression vector pcmv-e2f1
    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( <t>E2F1</t> , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.
    E2f1 Expression Vector Pcmv E2f1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pmc07509406-81-1-11
    Average 90 stars, based on 1 article reviews
    e2f1 expression vector pcmv-e2f1 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier
    e2f1 expression vector e2f1 ha  (Addgene inc)
    94
    Addgene inc e2f1 expression vector e2f1 ha
    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( <t>E2F1</t> , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.
    E2f1 Expression Vector E2f1 Ha, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pCMVHA+E2F1+(Plasmid+%2324225)/ppr0125839-93-9-17
    Average 94 stars, based on 1 article reviews
    e2f1 expression vector e2f1 ha - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    e2f1 expression vector  (Addgene inc)
    96
    Addgene inc e2f1 expression vector
    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( <t>E2F1</t> , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.
    E2f1 Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2f1+expression+vector/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pmc07086368__thnov10p3952s1-19-9-13
    Average 96 stars, based on 1 article reviews
    e2f1 expression vector - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    E2F1 upregulates LSINCT5 expression in GC cells. Notes: qRT-PCR analysis of the E2F1 expression levels in GC tissues and paired ANTs ( A ) and in metastatic and nonmetastatic GC tissues ( B ). ( C ) qRT-PCR analysis of E2F1 expression in a series of human GC cell lines and a normal gastric epithelial cell line (GES-1). ( D ) ChIP assays using E2F1 antibody demonstrated endogenous E2F1 binding to the LSINCT5 gene promoter, and ectopic expression or siRNA knockdown increased or reduced E2F1 enrichment at the LSINCT5 promoter, respectively. ( E ) A dual-luciferase reporter assay was performed through cotransfection of the LSINCT5 promoter fragment (pGL3-LSINCT5) and an E2F1-overexpression construct. ( F ) We examined the LSINCT5 core promoter region for transcription factor binding sites, and identified four tandem putative E2F1-binding sites. Reporter assays were conducted in cells transfected with various LSINCT5 promoter constructs in which different E2F1-binding elements were deleted (WT, del). Luciferase activity is presented relative to that of the pGL3 vector (a promoter-less vector). ( G ) qRT-PCR analysis of the LSINCT5 expression levels following the transfection of MGC803 and BGC823 cells with a pMax-E2F1 expression vector and siRNA-E2F1, respectively. ( H ) Analysis of the relationship between LSINCT5 and E2F1 mRNA levels (ΔCt value) in GC tissues. Bars: SD; * P <0.05 and ** P <0.01. Abbreviations: ANTs, adjacent normal tissues; Ts, tumor tissues; ChIP, chromatin immunoprecipitation; del, deletion; GC, gastric cancer; qRT-PCR, quantitative real-time polymerase chain reaction; WT, wild type; NC, negative control.

    Journal: Cancer Management and Research

    Article Title: E2F1 induces LSINCT5 transcriptional activity and promotes gastric cancer progression by affecting the epithelial-mesenchymal transition

    doi: 10.2147/CMAR.S171652

    Figure Lengend Snippet: E2F1 upregulates LSINCT5 expression in GC cells. Notes: qRT-PCR analysis of the E2F1 expression levels in GC tissues and paired ANTs ( A ) and in metastatic and nonmetastatic GC tissues ( B ). ( C ) qRT-PCR analysis of E2F1 expression in a series of human GC cell lines and a normal gastric epithelial cell line (GES-1). ( D ) ChIP assays using E2F1 antibody demonstrated endogenous E2F1 binding to the LSINCT5 gene promoter, and ectopic expression or siRNA knockdown increased or reduced E2F1 enrichment at the LSINCT5 promoter, respectively. ( E ) A dual-luciferase reporter assay was performed through cotransfection of the LSINCT5 promoter fragment (pGL3-LSINCT5) and an E2F1-overexpression construct. ( F ) We examined the LSINCT5 core promoter region for transcription factor binding sites, and identified four tandem putative E2F1-binding sites. Reporter assays were conducted in cells transfected with various LSINCT5 promoter constructs in which different E2F1-binding elements were deleted (WT, del). Luciferase activity is presented relative to that of the pGL3 vector (a promoter-less vector). ( G ) qRT-PCR analysis of the LSINCT5 expression levels following the transfection of MGC803 and BGC823 cells with a pMax-E2F1 expression vector and siRNA-E2F1, respectively. ( H ) Analysis of the relationship between LSINCT5 and E2F1 mRNA levels (ΔCt value) in GC tissues. Bars: SD; * P <0.05 and ** P <0.01. Abbreviations: ANTs, adjacent normal tissues; Ts, tumor tissues; ChIP, chromatin immunoprecipitation; del, deletion; GC, gastric cancer; qRT-PCR, quantitative real-time polymerase chain reaction; WT, wild type; NC, negative control.

    Article Snippet: The pMax-E2F1 expression vector was purchased from Addgene (plasmid #16007) (Cambridge, MA, USA), and siRNAs specific for E2F1 (#AM16708) and LSINCT5 (#4392420) were chemically synthesized (Invitrogen, Carlsbad, CA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Cotransfection, Over Expression, Construct, Transfection, Activity Assay, Plasmid Preparation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Negative Control

    Primers used in this study

    Journal: Cancer Management and Research

    Article Title: E2F1 induces LSINCT5 transcriptional activity and promotes gastric cancer progression by affecting the epithelial-mesenchymal transition

    doi: 10.2147/CMAR.S171652

    Figure Lengend Snippet: Primers used in this study

    Article Snippet: The pMax-E2F1 expression vector was purchased from Addgene (plasmid #16007) (Cambridge, MA, USA), and siRNAs specific for E2F1 (#AM16708) and LSINCT5 (#4392420) were chemically synthesized (Invitrogen, Carlsbad, CA, USA).

    Techniques: Real-time Polymerase Chain Reaction

    Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( E2F1 , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.

    Journal: Frontiers in Oncology

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    doi: 10.3389/fonc.2020.01730

    Figure Lengend Snippet: Expression analysis for genes that as the G1-S phase and DNA replication regulators after RPL21 knockdown. (A,B) The validation of transcriptome analysis by quantitative real-time PCR (qPCR). Human pancreatic cancer PANC-1 cells were transfected with siL21-Mix (40 nM, 72 h), the up-regulated genes ( AHR , THBS1 , DDIT3 , and MKNK2 ) in transcriptome sequencing were confirmed by qPCR. The down-regulated genes ( E2F1 , PCNA , CCND1 , CCNE1 MCM2 , MCM4 , MCM5 , MCM7 , and KIAA0101 ) in transcriptome sequencing were confirmed by qPCR. (C) The PANC-1 and BxPC-3 cells were transfected with Mock-siRNA, siL21-1 and siL21-2 (40 nM, 72 h). The equal amounts (15 μg) of each protein sample were analyzed by western blot with antibodies of E2F1 , CCND1 , CCNE1 , MCM2 -7 and GAPDH . The GAPDH served as an internal control. The control, negative control (NC), siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. Three independent experiments were of similar results.

    Article Snippet: The E2F1 expression vector pCMV-E2F1 was constructed using the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, United States).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transfection, Sequencing, Western Blot, Negative Control

    RPL21 regulates the cell cycle and DNA replication via E2F1 in PANC-1 and BxPC-3 cells. (A,C) The luciferase reporter vectors ( CCND1 , CCNE1 and MCM2 - 7 ) were constructed using pGL-3 vectors. 1 μg reporter vector was co-transfected with 1 μg pCMV-E2F1 vector or 1 μg empty pcDNA3.1 vector (control) for each well (6-well plates) containing 6 × 10 5 PANC-1 and BxPC-3 cells. Luciferase activity was measured 24 h after the transfection. (B,D) The PANC-1 and BxPC-3 cells were transfected with siRNAs with lipofectamine 2000 reagent. The control, NC, siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. The cells after transfection were seeded in 6-well plates at 6 × 10 5 cells/well, and 2 g E2F1 luciferase reporters (E2F1-promoter) were transfected using lipofectamine 2000 reagent. Luciferase activity was measured 24 h after the transfection. Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t -test.

    Journal: Frontiers in Oncology

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    doi: 10.3389/fonc.2020.01730

    Figure Lengend Snippet: RPL21 regulates the cell cycle and DNA replication via E2F1 in PANC-1 and BxPC-3 cells. (A,C) The luciferase reporter vectors ( CCND1 , CCNE1 and MCM2 - 7 ) were constructed using pGL-3 vectors. 1 μg reporter vector was co-transfected with 1 μg pCMV-E2F1 vector or 1 μg empty pcDNA3.1 vector (control) for each well (6-well plates) containing 6 × 10 5 PANC-1 and BxPC-3 cells. Luciferase activity was measured 24 h after the transfection. (B,D) The PANC-1 and BxPC-3 cells were transfected with siRNAs with lipofectamine 2000 reagent. The control, NC, siL21-1 and siL21-2 represented the untransfected, Mock-siRNA transfected, siL21-1 and siL21-2 transfected, respectively. The cells after transfection were seeded in 6-well plates at 6 × 10 5 cells/well, and 2 g E2F1 luciferase reporters (E2F1-promoter) were transfected using lipofectamine 2000 reagent. Luciferase activity was measured 24 h after the transfection. Each bar represents the mean ± SD of triplicate analysis. * indicates P < 0.05 compared to the control as determined by the Student’s t -test.

    Article Snippet: The E2F1 expression vector pCMV-E2F1 was constructed using the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, United States).

    Techniques: Luciferase, Construct, Plasmid Preparation, Transfection, Activity Assay

    Model of E2F1 -mediated pancreatic cancer cell proliferation.

    Journal: Frontiers in Oncology

    Article Title: RPL21 siRNA Blocks Proliferation in Pancreatic Cancer Cells by Inhibiting DNA Replication and Inducing G1 Arrest and Apoptosis

    doi: 10.3389/fonc.2020.01730

    Figure Lengend Snippet: Model of E2F1 -mediated pancreatic cancer cell proliferation.

    Article Snippet: The E2F1 expression vector pCMV-E2F1 was constructed using the pcDNA3.1 plasmid (Invitrogen, Carlsbad, CA, United States).

    Techniques: